Composite

Part:BBa_K2374006:Design

Designed by: Jianfeng Zhou   Group: iGEM17_Tongji_China   (2017-10-26)


TH-Gal80ts


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 479
    Illegal BglII site found at 1075
    Illegal BamHI site found at 137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 487
    Illegal BsaI site found at 533


Design Notes

In-Fusion Cloning


Source

TH promoter -> Drosophila melanogaster Gal80ts -> Saccharomyces cerevisiae

References